Phân tích đặc điểm di truyền ORF2 của Porcine circovirus type 2 (PCV2) thu thập ở một số Tỉnh/Thành Việt Nam trong giai đoạn từ 2007- 2016=Genetic characteristic analysis of ORF2 of Porcine circovirus type 2 (PCV2) in some provinces, Viet Nam, in the period 2007

Phân tích đặc điểm di truyền ORF2 của Porcine circovirus type 2 (PCV2) thu thập ở một số Tỉnh/Thành Việt Nam trong giai đoạn từ 2007- 2016=Genetic characteristic analysis of ORF2 of Porcine circovirus type 2 (PCV2) in some provinces, Viet Nam, in the period 2007 - 2016

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Tác giả: Dung Nguyễn Văn, Hải Nguyễn Ngọc, Hạnh Trần Xuân, Phúc Nguyễn Ngọc Hồng, Quách Võ Ngôn, Thu Hồng Nguyễn Thị, Thu Phương Lê Thi

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học kỹ thuật Thú y, 2018

Mô tả vật lý: tr.23

Bộ sưu tập: Metadata

ID: 498509

Thêm vào giỏ

Porcine circovirus type 2 (PCV2) is a causative agent relating to several porcine circovirus diseases- PCVDs, causing heavy economic losses in the swine industry. With a high nucleotide substitution rate,PCV2 continues to evolve and shift to novel genotypes. To determine the prevalence of PCV2 genotypesin Viet Nam, full-length of ORF2 of 48 PCV2 isolates collecting from 13 provinces in Viet Nam in theperiod from 2007 to 2016 was analysed to determine nucleotide sequence, build phylogenetic tree andcompare to PCV2 strains that reported in Viet Nam and other countries. The analysed results showedthat genotypes of PCV2b, PCV2d and recombinant cluster were co-existed and prevalent. Of which,the most common was PCV2b (24/48) and the emergence of PCV2d, especially PCV2d2 (15/16). Thenucleotide similarity level of the PCV2 isolates classifying in the same genotype was relatively high,such as: 98.7% - 100% for PCV2b, 98.5 - 100% for PCV2d2 and 98.7 - 100% for recombinant cluster.Genetic divergence among the genotypes was also quite high ranging from 0.0595 ± 0.0096 to 0.0663± 0.0102. Besides, genotype change by time had occurred at the farm level. These studied resultscontribute to further clarification of PCV2 epidemiology in Viet Nam.Porcine circovirus type 2 (PCV2) là tác nhân liên quan đến nhiều bệnh, gây thiệt hại nghiêm trọng trongchăn nuôi heo. PCV2 có tốc độ thay thế nucleotide nhanh tạo điều kiện để virus tiến hóa và xuất hiện cácgenotype mới. Sự lưu hành và biến đổi của các genotype PCV2 ở Việt Nam được đánh giá qua việc giải trình tựnucleotide toàn bộ ORF2 của 48 chủng PCV2 phân lập thực địa từ 13 tỉnh/thành, Việt Nam trong giai đoạn từnăm 2007 đến năm 2016, phân tích cây di truyền và so sánh mức tương đồng với các chủng PCV2 tham khảoở Việt Nam và trên thế giới. Kết quả phân tích cho thấy, có sự lưu hành đồng thời của các genotype PCV2b,PCV2d và nhóm tái tổ hợp, trong đó phổ biến nhất là PCV2b (24/48), cùng với sự xuất hiện và ngày càng phổbiến của PCV2d (16/48), đặc biệt là PCV2d2 (15/16). Các phân lập PCV2 xếp trong cùng genotype có mứcđộ tương đồng về nucleotide là khá cao, từ 98,7% - 100% đối với PCV2b, từ 98,5 – 100% đối với PCV2d2 vàtừ 98,7% đến 100% đối với nhóm PCV2 tái tổ hợp. Khoảng cách di truyền giữa các genotype là khá cao, biếnđộng từ 0,0595 ± 0,0096 đến 0,0663 ± 0,0102. Ngoài ra, có sự biến đổi genotype theo thời gian xảy ra ở mứcđộ trang trại. Kết quả này góp phần làm rõ hơn về dịch tễ học của PCV2 ở Việ

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