TUYỂN CHỌN VÀ KHẢO SÁT MÔI TRƯỜNG ẢNH HƯỞNG ĐẾN HOẠT TÍNH CHITINASE THU NHẬN TỪ NẤM MỐC = SELECTING AND INVESTIGATING THE EFFECTS OF ENVIRONMENT ON CHARACTERISTICS OF CHITINASE FROM SOIL FUNGI

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Tác giả: Trần Quỳnh Hoa, Khoa Nguyễn Đăng, Đào Thị Mỹ Linh, Nguyễn Thị Quỳnh Mai, Nga Sơn Thiên, Thu Bùi Thiên Kim, Nguyễn Đình Triều Vũ, Kiều Yến Vy

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học Công nghệ và Thực phẩm (Tên mới: Tạp chí Khoa học Đại học Công Thương), 2019

Mô tả vật lý: tr.38

Bộ sưu tập: NCBI

ID: 498571

Thêm vào giỏ

Chitinase is the second-largest enzyme group in the world after cellulase, it has wide applications in many fields of agriculture, industry, and medicine. Chitooligosaccharides, the chitin hydrolyzed products by chitinase, have many medical properties such as antibacterial activity, anti-cancer, antioxidant and immunostimulating. This study was conducted with the aim of selecting a fungus strain with the highest chitinase biosynthesis ability from the soil and investigating some factors affecting chitinase activity. The selection process was conducted by culturing the fungi on M0 medium for 14 days. We found a fungus strain with the best chitinase biosynthesis based on raw chitinase activity. This fungus strain was identified via the 28S rRNA gene sequencing as Aspergillus sydowii. The factors affecting the culture medium on chitinase activity were investigated including types of culture medium (MT1, MT2, MT3, MT4, MT5), chitin content (0-20%), seed rate of 1-3% (v/w), carbon source (glucose, starch, yellow sugar is 0.2, 0.4, 0.6 g/10.5g medium, respectively), culture time (24- 96 hours). The results showed that the good conditions for biosynthesis of chitinase from Aspergillus sydowii were deternined: MT4 medium with 1 mL seedling rate, percentage of chitin is 15%, culture time was 48 hours and carbon source was brown sugar with 0.6 g (w/w). Activity of obtained chitinase was 1,667 UI/mL. Keywords: Aspergillus sydowii, chitinase, chitin, carbon source. Chitinase thuộc nhóm enzyme lớn thứ hai trên thế giới sau cellulase, có nhiều ứng dụng rộng rãi trong nhiều lĩnh vực nông nghiệp, công nghiệp và y dược. Đặc biệt dẫn xuất COS - sản phẩm thủy phân chitin bởi chitinase có nhiều đặc tính y học như hoạt tính kháng khuẩn, tác dụng chống ung thư, chống oxy hóa và hoạt động kích thích miễn dịch. Nghiên cứu được thực hiện với mục đích tuyển chọn chủng nấm có khả năng sinh tổng hợp chitinase cao nhất và khảo sát một số yếu tố ảnh hưởng đến hoạt tính chitinase. Quá trình tuyển chọn được tiến hành trên môi trường M0, sau 14 ngày nuôi cấy thu dịch enzyme thô, chủng nấm mốc có hoạt tính chitinase cao nhất được định danh bằng phương pháp giải trình tự gen 28S rRNA. Các yếu tố ảnh hưởng của môi trường tới hoạt tính chitinase được khảo sát bao gồm các loại môi trường nuôi cấy (MT1, MT2, MT3, MT4, MT5), tỷ lệ chitin 0-20% (w/w), tỷ lệ giống 1-3% (v/w), nguồn cacbon (glucose, tinh bột, đường vàng) tỷ lệ tương ứng 0,2
0,4
0,6 g (w/w), thời gian nuôi cấy (24-96 giờ). Kết quả cho thấy môi trường MT4 với tỷ lệ giống cấy 1 mL, tỷ lệ cơ chất chitin 15%, thời gian nuôi cấy 48 giờ và đường vàng bổ sung với hàm lượng 0,6 g (w/w) là tốt nhất cho sinh tổng hợp enzyme chitinase từ Aspergillus sydowii có hoạt tính đạt 1,667 UI/mL. Từ khóa: Aspergillus sydowii, chitinase, chitin, nguồn cacbon.

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