Kết quả phân lập xác định đặc tính sinh học và nhóm kháng huyết thanh của các chủng Salmonella SPP. ở thịt lợn tại tỉnh Lạng Sơn = Isolation, biological charateristic and antisera determination of Salmonella spp in pork in Lang Son province

0 Người đánh giá. Xếp hạng trung bình 0

Tác giả: Nguyễn Thị Ngân, Tuyên Nguyễn Văn

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học kỹ thuật Thú y, 2018

Mô tả vật lý: tr.49

Bộ sưu tập: NCBI

ID: 498602

Thêm vào giỏ

Kết quả phân lập và xác định một số đặc điểm sinh học, nhóm kháng huyết thanh của các chủngSalmonella spp. nhiễm trong thịt lợn thu thập từ một số chợ bán lẻ tại tỉnh Lạng Sơn cho thấy:- Xét nghiệm 90 mẫu thịt lợn thu tại các chợ (Giếng Vuông, Chi Lăng, Đông Kinh), đã phát hiệnđược 12,22% số mẫu nhiễm Salmonella spp. trong đó tỷ lệ mẫu thịt nhiễm cao nhất là ở chợ Chi Lăng(16,67%), tiếp theo là ở chợ Đông Kinh (13,33%) và thấp nhất là ở chợ Giếng Vuông (6,67%).- Tỷ lệ mẫu thịt nhiễm khuẩn Salmonella spp. tăng dần theo thời gian sau giết mổ và từ mùa đông(tháng 10) đến mùa hè.- Kết quả kiểm tra mức độ mẫn cảm với kháng sinh của 6 chủng vi khuẩn phân lập cho thấy có 83,33%mẫn cảm với ciprofloxacine, 66,67% mẫn cảm với enrofloxacine, 50% mẫn cảm với norfloxacine. Cácchủng Salmonella phân lập được đều kháng với tetracycline. Các chủng vi khuẩn này đều có độc lựcrất cao, gây chết 100% động vật thí nghiệm trong vòng 24 - 48 giờ sau khi tiêm.- Vi khuẩn Salmonella spp. phân lập được mang đầy đủ các đặc tính sinh vật, hóa học của giốngSalmonella. Trong đó có 5 chủng (45,45%) đã được xác định thuộc S. typhimurium, 3 chủng (27,27%)thuộc S. weltevreden, 2 chủng (18,18%) thuộc S. anatum, 1 chủng (9,09%) thuộc S. schlleissheim. The result of isolation and determination of the biological characteristics and antisera groupsof Salmonella spp. in pork in Lang Son province indicated that out of 90 pork samples collectingin Lang Son (Gieng Vuong, Chi Lang, Dong Kinh pork retail markets), there were 12.22% ofsamples contaminated with Salmonella spp. Of which, the highest contamination rate was in ChiLang market (16.67%), followed by Dong Kinh market (13.33%) and the lowest contaminationrate was in Gieng Vuong market (6.67%). The Salmonella contamination rate of pork increasedby the time after slaughter and seasons from winter to summer. The result of testing susceptibilitywith antibiotics of 6 isolated bacteria strains indicated that the susceptible rate of bacteria strainwith ciprofloxacine was 83.33%, with enrofloxacine was 66.6%, with norfloxacine was 50%. Allof isolates resisted with tetracycline, and they were the high virulent strains, killing 100% of theexperimental animals within 24 – 48 hrs after injection. The isolates carried all the biological andchemical characteristics of Salmonella bacteria genus, and the strains were determined, such as:5 strains belonged to S. typhimurium (45.45%)
3 strains belonged to S. welteveden (27.27%)
2strains belonged to S. anatum (18.18%) and 1 strain belonged to S. schlleissheim (9.09%)

Tạo bộ sưu tập với mã QR