Nghiên cứu đa hình đơn nucleotide rs36084323 của gen PDCD-1 ở bệnh nhân nhiễm vi rút viêm gan B mạn tính

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Tác giả: Thị Ngọc Dung Đặng, Phương Giang Đào, Xuân Hoàn Nghiêm, Thị Uyên Ngọ, Thị Minh Huyền Phạm, Thành Văn Tạ

Ngôn ngữ: vie

Ký hiệu phân loại: 616.97042 Other diseases

Thông tin xuất bản: Tạp chí Nghiên cứu y học (Đại học Y Hà Nội), 2022

Mô tả vật lý: 25-35

Bộ sưu tập: Metadata

ID: 498669

Thêm vào giỏ

Đa hình đơn nucleotide (SNP) của gen PDCD-1 được cho là có liên quan đến sự thay đổi phiên mã PD-1 là phối tử quan trọng tham gia ức chế điểm kiểm soát miễn dịch tế bào T vì vậy có thể đóng vai trò quan trọng trong quá trình sinh lý bệnh của bệnh viêm gan B mạn tính. Nghiên cứu mô tả cắt ngang gồm 298 bệnh nhân nhiễm vi rút viêm gan B (HBV) mạn tính [133 bệnh nhân viêm gan B mạn tính (CHB), 165 ung thư biểu mô tế bào gan (HCC)] và 159 người khỏe mạnh (HC) được tiến hành xác định tỉ lệ kiểu gen tại locus SNP rs36084323 của gen PDCD-1 bằng phương pháp giải trình gen Sanger sequencing và xác định mối liên quan giữa SNP rs36084323 với nguy cơ nhiễm vi rút HBV mạn tính và ung thư gan. Kết quả cho thấy tỉ lệ kiểu gen CC, CT, TT lần lượt ở nhóm HC là 31,4%, 49,7%, 18,9%, ở nhóm CHB là 35,3%, 51,9%, 12,8%, ở nhóm HCC là 30,9%, 50,3%, 18,8%. Phân tích mô hình di truyền trội, so sánh tỉ lệ kiểu gen CC và CT với kiểu gen TT giữa các nhóm: nhiễm HBV mạn tính và HC: OR 1,21, 95%CI = 0,73 - 2,00, p >
0,05
HCC và CHB: OR = 1,58, 95%CI = 0,83 - 3.00, p >
0,05. Kết quả này cho thấy, SNP rs36084323 của gen PDCD-1 không liên quan đến nguy cơ nhiễm vi rút viêm gan B và không làm tăng nguy cơ tiến triển viêm gan B mạn tính thành ung thư gan.The single nucleotide polymorphisms (SNPs) of the PDCD-1 gene are thought to be involved in transcriptional changes of PD-1, which is an important ligand involved in T-cell depletion and therefore may play a role important role in the pathophysiology of chronic Hepatitis B. We designed a cross sectional study- recruited 298 patients with chronic HBV infection [133 patients with chronic hepatitis B (CHB) and165 patients with hepatocellular cancer (HCC)] and 159 healthy individuals (HC). PD1.1 was genotyped by Sanger sequencing method. We analyzed the association between SNP rs36084323 and HBV infection susceptibility and HCC progression risk. The frequencies of genotype at SNP rs36084323 CC,CT, and TT were: 31.4%, 49.7% 18.9% in the HC group
35.3%, 51.9%, and 12.8% in the CHB group, and 30.9%, 50.3%, and 18.8% in HCC group. There were no association between rs36084323 and HBV infection susceptibility and HCC development risk in the recessive model (TT vs. CC+CT): HBV infection vs. HC: OR = 1.21, 95% CI = 0.73 - 2.00, P >
0.05 and HCC vs. CHB: OR = 1.58, 95% CI = 0.83 - 3.00, P >
0.05. Result suggests that the SNP rs36084323 of the PDCD-1 gene did not associate with hepatitis B infection and HCC predisposition.

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