Using combined chromatographic methods, three flavonoids, (2S)-7,4'- dihydroxy-5-methoxyflavanone (1), luteolin (2), and 2¢¢-O-rhamnosylvitexin (3) were isolated from the methanol extract of the leaves of Vitex trifolia. Their structures were established on the basis of spectroscopic evidence and comparison with those reported in the literature.