Ảnh hưởng của thực khuẩn thể đến mật độ và hình thái khuẩn lạc vi khuẩn Vibrio spp.

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Tác giả: Thị Mỹ Ảnh Đỗ, Hoàng Bảo Ngọc Lê, Thị Kim Thoa Nguyễn, Hoài An Phạm, Kim Ngân Phan, Trần Học Khang Phan, Ngọc Thạnh Tiêu, Hữu Hậu Trần, Ngọc Thảo Vy Trần, Thị Bích Vân Trương, Ngọc Trân Anh Võ

Ngôn ngữ: vie

Ký hiệu phân loại: 612.803 Human physiology

Thông tin xuất bản: Khoa học Nông nghiệp Việt Nam, 2022

Mô tả vật lý: 751-756

Bộ sưu tập: Metadata

ID: 498976

Thêm vào giỏ

Nghiên cứu đã đánh giá sự ảnh hưởng của thực khuẩn thể lên kiểu hình và mật độ của vi khuẩn Vibrio spp. Nguồn vi khuẩn thuộc nhóm Vibrio spp. được phân lập tại xã An Thạnh 2 và xã An Thạnh Đông, huyện Cù Lao Dung, tỉnh Sóc Trăng và 18 dòng thực khuẩn thể cũng được cung cấp bởi Phòng Thí nghiệm Sinh học Phân tử, Viện Nghiên cứu và Phát triển Công nghệ Sinh học, Trường Đại học Cần Thơ. Nghiên cứu đã sử dụng phương pháp trải đếm để quan sát số lượng và những thay đổi về hình thái khuẩn lạc của vi khuẩn khi cho tương tác giữa thực khuẩn thể và Vibrio spp. Kết quả cho thấy những thay đổi về số lượng và kiểu hình khuẩn lạc của từng dòng thực khuẩn thể khác nhau. Cụ thể 7 dòng thực khuẩn thể là F2, F3, F4, F5, F7, F8, và F22 làm giảm rõ rệt số lượng khuẩn lạc của vi khuẩn Vibrio sp. B1.1. Tuy nhiên, 4 dòng thực khuẩn thể F1, F19, F21, và F23 lại làm tăng lượng khuẩn lạc của vi khuẩn. Trong thí nghiệm quan sát sự thay đổi hình thái đáng chú ý có dòng thực khuẩn thể F21, F22, và F23 làm thay đổi màu sắc, kích thước và bìa của khuẩn lạc vi khuẩn Vibrio sp. B1.1. Thực khuẩn thể có có khả năng làm thay đổi số lượng và kiểu hình khuẩn lạc của vi khuẩn.The study was condcuted to evaluate the effects of phages on the phenotype and density of Vibrio spp. The Vibrio spp. strains were isolated from An Thanh 2 and An Thanh Dong commune, Cu Lao Dung district, Soc Trang province, and 18 bacteriophage strains were obtained from the Molecular Biology Laboratory, Biotechnology Research and Development Institute, Can Tho University. The spread counting method was employed to observe the number and changes in colony morphology of Vibrio spp. after treatment with bacteriophages. The results showed that there were changes in the number and phenotype of colonies of the tested bacteria with different phage strains. Specifically, seven bacteriophage strains F2, F3, F4, F5, F7, F8, and F22 significantly reduced the number of colonies of Vibrio sp. strain B1.1 while four bacteriophages F1, F19, F21, and F23 increased the number of bacterial colonies. In addition, remarkable morphological changes were observed in color, size and cover of Vibrio sp. strain B1.1 after treating with phage F21, F22, and F23. The findings indicated that bacteriophages exerted the ability to change the number and phenotype of Vibrio spp. colonies.

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