Đặc điểm khối tế bào gốc phân lập từ tủy xương tự thân hỗ trợ điều trị bệnh teo đường mật bẩm sinh

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Tác giả: Thị Hà Đặng, Thị Phương Hà, Đức Minh Lê, Thị Nghiêm Lương, Bảo Ngọc Nguyễn, Phạm Anh Hoa1 Trần Minh Điển1 Nguyễn, Thanh Bình Nguyễn, Thị Duyên Nguyễn, Duy Hiền Phạm

Ngôn ngữ: vie

Ký hiệu phân loại: 174.25 Innovative procedures

Thông tin xuất bản: Tạp chí Nghiên cứu y học (Đại học Y Hà Nội), 2022

Mô tả vật lý: 20-27

Bộ sưu tập: Metadata

ID: 499858

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Biliary atresia is one of the most common causes of persistent jaundice in the neonatal period. Treatment of this condition still remains challenging with poor prognosis. This study was carrried out to describe characteristics of autologous bone marrow stem cell unit derived from bone marrow samples for supportive treatment of biliary atresia. We conducted a case series of 30 patients diagnosed with biliary atresia and undergone Kasai surgery combined with stem cell therapy at Vietnam National Children's Hospital. The results showed that in bone marrow stem cell units, the average number of total nucleated and mononuclear cells was 0.88 ± 0.28 × 109 cells and 0.51 ± 0.16 ×109 cells, respectively. The average number of hematopoietic stem cells and mesenchymal stem cells was 40.32 ± 17.63 × 106 cells and 118.2 ± 17.71 × 103 cells. The dose of mononuclear cells, hematopoietic stem cells and mesenchymal stem cells injected to patients was 0.1× 109, 7.38 × 106 and 3.13 × 103 cells/kg body weight, respectively. All bone marrow stem cell units have the standard criteria for supportive treatment of biliary atresia patients.Teo đường mật bẩm sinh là một trong những nguyên nhân phổ biến nhất gây vàng da kéo dài ở trẻ sơ sinh. Điều trị bệnh còn khó khăn và tiên lượng thường xấu. Nghiên cứu được thực hiện với mục tiêu mô tả đặc điểm khối tế bào gốc phân lập từ dịch tủy xương hỗ trợ điều trị bệnh teo đường mật bẩm sinh ở trẻ em. Phương pháp nghiên cứu mô tả cắt ngang trên 30 bệnh nhân chẩn đoán teo đường mật bẩm sinh được điều trị phẫu thuật Kasai kết hợp với truyền tế bào gốc tủy xương tự thân trong mổ tại Bệnh viện Nhi Trung ương. Kết quả cho thấy, khối tế bào gốc tủy xương tự thân có tổng tế bào có nhân và tế bào đơn nhân trung bình lần lượt là 0,88 ± 0,28 × 109 tế bào và 0,51 ± 0,16 ×109 tế bào. Số lượng tế bào gốc tạo máu và tế bào gốc trung mô là 40,32 ± 17,63 × 106 tế bào và 18,2 ± 17,71 × 103 tế bào. Liều truyền tế bào đơn nhân, tế bào gốc tạo máu CD34+ và tế bào gốc trung mô lần lượt là 100 × 106 tế bào/kg cân nặng, 7,38 × 106 tế bào/kg cân nặng và 3,13 × 103 tế bào/kg cân nặng. Các khối tế bào gốc tủy xương tự thân thu được đảm bảo về số lượng, mật độ và liều truyền của các loại tế bào sử dụng trong liệu pháp hỗ trợ điều trị cho bệnh nhân teo đường mật bẩm sinh.

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