Nghiên cứu độc lực của các chủng Salmonella phân lập được từ gà mắc bệnh thương hàn và sử dụng chế phẩm NanoSan trong điều trị bệnh cho gà tại huyện Tam Dương, tỉnh Vĩnh Phúc

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Tác giả: Thị Hồng Duyên Dương, Thị Ngân Nguyễn, Xuân Yên Nguyễn, Diệu Thùy Phạm, Thị Hồng Phúc Phan, Nhật Thắng Trần

Ngôn ngữ: vie

Ký hiệu phân loại: 571.993 Physiology and related subjects

Thông tin xuất bản: Khoa học và Công nghệ - Đại học Thái Nguyên, 2022

Mô tả vật lý: 159-166

Bộ sưu tập: Metadata

ID: 500192

Thêm vào giỏ

Nghiên cứu này khảo sát tỷ lệ mắc tiêu chảy trên đàn gà nuôi tại các nông hộ và trang trại thuộc 3 xã: Thanh Vân, Duy Phiên, Kim Long của huyện Tam Dương, tỉnh Vĩnh Phúc. Kết quả cho thấy, có 3,69% gà mắc tiêu chảy ở giai đoạn 1-6 tuần tuổi, 4,10% ở 7-20 tuần tuổi và 6,55% ở gà trên 20 tuần tuổi
có 6,60% gà mắc tiêu chảy ở vụ Xuân Hè và 2,89% gà mắc tiêu chảy ở vụ Thu Đông
6,70% gà nuôi chuồng hở và 2,92% gà nuôi chuồng kín mắc tiêu chảy. Kiểm tra độc lực của 20 chủng vi khuẩn Salmonella gallinarum pullorum phân lập được bằng phản ứng PCR có 20/20 chủng (100%) mang gen Stn và gen InvA
không có chủng nào mang yếu tố DT104. Sử dụng ngẫu nhiên 3 chủng vi khuẩn Salmonella gallinarum pullorum tiêm cho 6 chuột nhắt trắng, cả 6/6 (100%) chuột chết từ 8-20 giờ sau tiêm. Kết quả thử nghiệm kháng sinh đồ cho thấy, thuốc norfloxacin và enrofloxacin mẫn cảm nhất với vi khuẩn Salmonella (có đường kính vòng vô khuẩn trung bình đạt 26 - 34 mm). Sử dụng thuốc enrofloxacin kết hợp với chất điện giải (gluco K-C thảo dược) điều trị cho gà mắc bệnh thương hàn có 90,00% gà khỏi bệnh
sử dụng thuốc enrofloxacin kết hợp với chất điện giải (gluco K-C thảo dược) và chế phẩm NanoSan điều trị cho gà mắc bệnh thương hàn có 93,33% gà khỏi bệnh.This study investigates the prevalence of diarrhea in chickens raised in households and farms in 3 communes: Thanh Van, Duy Phien, Kim Long of Tam Duong district, Vinh Phuc province. The results showed that, 3.69% of chickens had diarrhea at 1-6 weeks old, 4.10% at 7-20 weeks old and 6.55% of chickens over 20 weeks old
6.60% of chickens had diarrhea in the spring-summer season and 2.89% of chickens had diarrhea in the autumn-winter season
6.70% of chickens raised in open cages and 2.92% of chickens in closed cages have diarrhea. Testing the virulence of 20 strains of Salmonella gallinarum pullorum isolated by PCR method, there were 20/20 strains (100%) carrying Stn and InvA genes
none of the strains carried the DT104 factor. Using randomly 3 strains of Salmonella gallinarum pullorum to be injected into 6 white mice, all 6/6 (100%) mice died from 8 to 20 hours after injection. The results of the antibiogram test showed that norfloxacin and enrofloxacin were most sensitive to Salmonella bacteria (the average antibiotic disk varied from 26 to 34 mm). Using enrofloxacin in combination with electrolytes (herbal K-C glucose) to treat salmonellosis, there was 90.00% of chickens recovered from the disease
using enrofloxacin in combination with electrolytes (herbal K-C glucose) and NanoSan probiotics to treat salmonellosis, there was 93.33% of chickens recovered from the disease.

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