Đặc điểm dấu ấn sinh học và bất thường gen MYC, BCL2, BCL6 trong bệnh u lympho không Hodgkin não nguyên phát tế bào B lớn lan tỏa tại Bệnh viện Chợ Rẫy

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Tác giả: Thị Thuý Hà Hoàng, Hùng Phong Lê, Hồng Phước Mạc, Trường Sơn Nguyễn, Quang Vinh Phạm

Ngôn ngữ: vie

Ký hiệu phân loại: 616.4 Diseases of hematopoietic, lymphatic, glandular systems Diseases of endocrine system

Thông tin xuất bản: Tạp chí Y học Việt Nam (Tổng hội Y học Việt Nam), 2022

Mô tả vật lý: 298-304

Bộ sưu tập: Metadata

ID: 500789

Thêm vào giỏ

Primary cerebral diffuse large B cell lymphoma is an uncommon variant of extranodal non - Hodgkin lymphoma. The cell origin of the tumor is the non- geminal center Bcell. Subjects and Research method: Subject: We performed a analysis of molecular biomarkers, MYC translocations, BCL2 and BCL6 rearrangements by FISH in 57 patients who have been diagnosed primary cerebral lymphoma. Research method: prospective descriptive study. Results: CD10, BCL-6, MUM1, BCL-2 antigens were immunohistochemically labeled on paraffin-embedded sections
the 4 were positive in 10,5%
61,4%
87,7% and 67,3% of the tumors, respectively. Among the 57 patients, the percentage of GCB was 19,3% and of non- GCB was 80,7%. The Ki67 index positive was high level (median 60%, range: 0-95%). The most common genetic abnormalities were BCL6 rearrangements (9,8%), MYC translocations (5,9%). BCL2 rearrangements and double-hit (MYC/BCL2, MYC/BCL6) occurred in 1 patient (1,9% each). Conclusion: The majority of primary cerebral lymphoma patients were classified in the non- GCB subgroup and the MYC translocations had the highest rate of genetic abnormalities.U lympho không Hodgkin (ULKH) não nguyên phát tế bào B lớn lan tỏa là một biến thể hiếm gặp của ULKH ngoài hạch. Nguồn gốc tế bào của khối u thuộc nhóm không tâm mầm với dấu ấn đặc trưng MUM1 và BCL6 dương tính tỷ lệ cao. Đối tượng và phương pháp nghiên cứu: Đối tượng gồm 57 bệnh nhân được chẩn đoán ULKH não nguyên phát tế bào B lớn lan tỏa, được làm xét nghiệm hóa mô miễn dịch và phát hiện bất thường gen MYC, BCL2, BCL6 bằng kỹ thuật FISH. Phương pháp nghiên cứu: mô tả tiến cứu. Kết quả: Tỷ lệ dương tính với dấu ấn CD10, BCL6, MUM1 và BCL2 là 10,5%
61,4%
87,7% và 67,3%. Tỷ lệ nhóm tâm mầm là 19,3% và không tâm mầm là 80,7%. Chỉ số Ki67 dương tính cao với trung vị 60% (từ 0% đến 95%). Bất thường di truyền thường gặp nhất là tái sắp xếp BCL6 (9,8%), chuyển đoạn MYC (5,9%). Tái sắp xếp BCL2 và double-hit (MYC/BCL2, MYC/BCL6) gặp ở 1 bệnh nhân (1,9% mỗi nhóm). Kết luận: Đa số bệnh nhân u lympho não nguyên phát có nguồn gốc tế bào không tâm mầm tái sắp xếp gen MYC gặp tỷ lệ cao nhất trong các bất thường di truyền.

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