A screen-printed carbon electrode (SPCE) based on a molecularly imprinted polymer (MIP) technique has been modified to provide detection of erlotinib (ERL). For this purpose, the pyrogallol (PG) monomer in the presence of ERL as an analyte has been electropolymerized on the SPCE surface. The introduced sensor has been characterized through differential pulse voltammetry (DPV), cyclic voltammetry (CV), and field emission scanning electron microscopy (FESEM) methods. The modified SPCE allowed ERL detection in a linear range of 0.05-800 nM with a limit of detection (LOD) of 0.016 nM. The applicability of modified SPCE was successfully investigated in serum samples. Lastly, for validation, sensor performance was compared with high-performance liquid chromatography (HPLC). The introduced sensor exhibited good application in the biomedical field.