Nghiên cứu mô hình suy giảm sinh sản trên chuột nhắt trắng đực gây béo phì bằng chế độ ăn giàu lipid

0 Người đánh giá. Xếp hạng trung bình 0

Tác giả: Thị Diễm Tiên Lê, Hoàng Tín Nguyễn, Minh Tiến Nguyễn, Phúc Duy Nguyễn, Văn Luân Nguyễn, Minh Thư Phùng, Thái Thanh Tâm Trần, Thúy Huỳnh Trần

Ngôn ngữ: vie

Ký hiệu phân loại: 636.708932 Dogs

Thông tin xuất bản: Tạp chí Y học Việt Nam (Tổng hội Y học Việt Nam), 2023

Mô tả vật lý: 324-328

Bộ sưu tập: Metadata

ID: 502359

Thêm vào giỏ

Đánh giá hình thái cơ quan sinh dục và chức năng sinh tinh của chuột nhắt trắng đực béo phì. Đối tượng và phương pháp: Chuột nhắt trắng đực (Swiss albino) được chia thành 2 lô (12 con/lô) gồm: lô NFD (Normal-fat diet) và lô HFD (High-fat diet). Sau 6 tuần gây béo phì, chuột được thực hiện đánh giá các chỉ số gồm khối lượng các cơ quan sinh dục, tinh dịch đồ, nồng độ testosteron huyết thanh, đường kính ống sinh tinh. Kết quả: Chuột ở lô HFD có khối lượng tinh hoàn và mào tinh thấp hơn lô NFD (p<
0,05). Tỷ lệ tinh trùng tiến tới, không tiến tới và mật độ tinh trùng lô HFD thấp hơn lô NFD (p<
0,001). Ngoài ra, tỷ lệ tinh trùng bất động và tinh trùng dị dạng lô HFD cao hơn lô NFD (p<
0,001). Nồng độ testosteron huyết thanh của chuột lô HFD cao hơn lô NFD 150% (p=0,001). Chuột lô HFD có đường kính ống sinh tinh thấp hơn chuột lô NFD 8,35% (p<
0,05). Kết luận: Sau 6 tuần gây mô hình bằng chế độ ăn giàu lipid, chuột béo phì có biểu hiện suy giảm sinh sản.To evaluate the genital morphology and spermatogenesis process in obese male mice. Materials and method: Male mice (Swiss albino) were divided into two groups, (12 animals/group), the NFD group (Normal-fat diet) and the other for the HFD group (High-fat diet). Mice were examined for markers such as the weight of sex organs, semen analysis, serum testosterone levels, and seminal vesicle diameters after 6 weeks of obesity induction. Results: The testes and epididymis of mice in the HFD group were smaller than those in the NFD group (p<
0.05). The HFD group's sperm concentration and the percentage of non-progressive and progressive sperms were lower than those of the NFD group (p<
0.001). Additionally, the HFD group showed a higher proportion of immotile sperm and abnormal morphology of sperm than that of NFD group (p<
0.001). The serum testosterone levels in the HFD group were 150% higher than those in the NFD group (p=0.001). The seminal vesicle diameters of mice in the HFD group was 8.35% smaller than that of mice in the NFD group (p<
0.05). Conclusion: Obese mice displayed reproductive impairment after 6 weeks of modeling with a high-fat diet.

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