Nhân nhanh giống sen Mặt Bằng (Nelumbo nucifera) bằng hệ thống nuôi cấy mô và vi thuỷ canh

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Tác giả: Thị Thu Hương Bùi, Thị Xuân Đỗ, Huy Giới Đồng, Hữu Cường Nguyễn, Thị Bích Lưu Nguyễn

Ngôn ngữ: vie

Ký hiệu phân loại: 571.538 *Tissue culture

Thông tin xuất bản: Khoa học và Công nghệ Lâm nghiệp, 2023

Mô tả vật lý: 51-60

Bộ sưu tập: Metadata

ID: 502402

Thêm vào giỏ

Nghiên cứu này sử dụng phương pháp nuôi cấy in vitro và vi thủy canh để nhân nhanh giống cây sen Mặt Bằng (tên khoa học), có nguồn gốc từ xã Sơn Đà, huyện Ba Vì, thành phố Hà Nội. Kết quả cho thấy, môi trường MS có 7,5 g/l agar, 30 g/l saccharose, 0,5 mg/l BAP là phù hợp nhất cho sự nảy mầm của phôi hạt sen với tỉ lệ phôi nảy mầm đạt 95%, chiều cao trung bình chồi 3,08 (cm) và số lá trung bình đạt 2,31 lá/chồi sau 7 ngày. Việc bổ sung kinetin hay BAP đều kích thích nhân chồi in vitro và vi thủy canh
đặc biệt môi trường MS bổ sung 1,5 mg/l BAP khiến 100% chồi tạo chồi mới, hệ số nhân 3,35 và 2,78 ở hệ thống nuôi cấy in vitro và vi thuỷ canh. Sự ra rễ của chồi sen đều tốt ở cả in vitro và vi thủy canh
và trong môi trường MS bổ sung 1,5 mg/l α- NAA thì 100% chồi ở hai hệ thống đều tạo rễ, với số rễ trung bình 11,63 rễ/cây, chiều dài rễ trung bình 3,10 cm ở hệ thống in vitro
và 9,78 rễ/cây, chiều dài rễ trung bình 2,08 cm ở hệ thống vi thủy canh. Giai đoạn thích ứng tự nhiên, cây có nguồn gốc vi thuỷ canh có tỉ lệ sống và số lá trung bình cao hơn cây in vitro ở các giá thể với tỷ lệ bùn và nước khác nhau. Giá thể 20 ml bùn: 40 ml nước thích hợp nhất cho cây sen nguồn gốc vi thuỷ canh giai đoạn thích nghi với tỷ lệ cây sống là 36,67% và số lá trung bình là 7,42 lá.This research used tissue culture and micro-hydroponics for plant propagation of the Mat Bang lotus. The result showed that (i) the embryos separated from the lotus seeds cultured on the MS medium with 7.5 g. L-1 agar + 30 g. L-1 saccharose and 0.5 mg/l BAP in vitro system was the best with an embryo germination rate of 64.4%, shoot height of 3.08 cm, and 2.31 leaves after 7 days of culture. (ii) During the shoot multiplication stage, the MS medium with Kinetin or BAP stimulated the lotus shoot multiplying in both in vitro and microponic systems. In particular, the MS added 1.5 mg. L-1 BAP made 100% of the shoots produce new shoots with a coefficient of 3.35 and 2.78 in vitro and microponic systems, respectively. (iii) The rooting of the shoots was available in the two systems. Specially, in the MS medium supplemented with 1.5 mg. L-1 α-NAA, 100% of samples produced roots, in which the average number of roots was 11.63 and root length averaged 3.10 cm in the in vitro system
and 9.78 roots/plant, the average root length was 2.08 cm in the microponic system. (iv) In the nursery, the microponic plantlets had higher survival rates and the average number of leaves was higher than those of in vitro plantlets in all substrates with different ratios of sludge and water. The substrate with the ratio of 20 ml of sludge and 40 ml of water was the most suitable for micro-hydroponic plantlets in the nursery with a survival rate of 36.67% and an average number of leaves of 7.42.

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