Phân lập và tuyển chọn chủng streptomyces có khả năng phân hủy lông gia cầm hiệu quả

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Tác giả: Hoang Trung Hieu Nguyen, Thi Dong Phuong Nguyen, Tran Huyen Anh Nguyen

Ngôn ngữ: vie

Ký hiệu phân loại: 636.61 Birds raised for feathers

Thông tin xuất bản: Tạp chí Khoa học và Công nghệ - Đại học Đà Nẵng, 2023

Mô tả vật lý: 31-34

Bộ sưu tập: Metadata

ID: 502426

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Feathers cause environmental problems worldwide when poultry processing industries produce it as a waste of billions of tons yearly. This waste is not easily decomposed by natural agents in a short time. Currently, Streptomyces spp. are known as a highly enzymatic activity species which can efficiently degrade feathers to products. Therefore, the aim of this study was to screen for Streptomyces strains with high feather-degrading activity. A total of 46 strains of Streptomyces spp. were isolated from different soils of Danang city and other vicinities, in which 13 isolates showed the ability to degrade casein and gelatin. The protease activity of H3, N5, and N7 isolates were higher than others. In particular, N7 strain had the highest protease activity with 70.33 U/mL and degraded 97.79% feather after 5 days of cultivation. Based on the sequence analysis of 16S rRNA, N7 was identified as homologous to Streptomyces anandii.Hằng năm các ngành công nghiệp chế biến gia cầm thải ra hàng tỷ tấn lông gia cầm và gây ra các vấn đề về môi trường trên toàn thế giới. Loại rác thải này khó bị phân hủy bởi các tác nhân tự nhiên trong thời gian ngắn. Hiện nay, Streptomyces spp. được biết đến như một loài có hoạt tính enzyme cao, có thể phân hủy lông gia cầm một cách hiệu quả. Vì vậy, mục đích của nghiên cứu này là sàng lọc các chủng Streptomyces có hoạt tính phân hủy lông gia cầm cao. Tổng cộng có 46 chủng Streptomyces được phân lập từ các loại đất khác nhau của thành phố Đà Nẵng và các vùng lân cận, trong đó có 13 chủng phân lập có khả năng phân hủy casein và gelatin. Hoạt tính protease của các chủng H3, N5, N7 cao hơn các chủng khác. Đặc biệt, chủng N7 có hoạt tính protease cao nhất với 70,33 U/mL và phân hủy 97,79% lông sau 5 ngày nuôi cấy. Dựa trên phân tích trình tự của 16S rRNA, N7 được xác định là tương đồng với loài Streptomyces anandii.

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