Tối ưu một số yếu tố ảnh hưởng đến quá trình tách chiết hợp chất polyphenol từ hạt cau (Areca catechu L.) bằng phương pháp đáp ứng bề mặt

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Tác giả: Thị Huyền Lê, Bá Thọ Nguyễn, Thị Liên Nguyễn, Minh Ngọc Trương

Ngôn ngữ: vie

Ký hiệu phân loại: 664.8 Fruits and vegetables

Thông tin xuất bản: Khoa học & Công nghệ Việt Nam, 2023

Mô tả vật lý: 48-53

Bộ sưu tập: Metadata

ID: 502465

Thêm vào giỏ

Polyphenol là một nhóm hợp chất tự nhiên có trong thực vật với đặc tính kháng khuẩn và chống ôxy hóa, mang lại nhiều tiềm năng ứng dụng trong sản xuất dược phẩm và nông nghiệp. Với mục đích tách chiết và thu nhận hàm lượng hợp chất polyphenol cao từ hạt cau (Areca catechu L.), nghiên cứu đã sử dụng phương pháp đáp ứng bề mặt để đánh giá ảnh hưởng của một số yếu tố kỹ thuật như nồng độ dung môi (X1), tỷ lệ dung môi/nguyên liệu (DM/NL) (v/w) (X2) và thời gian ngâm (giờ) (X3) đến hàm lượng hợp chất polyphenol (Y) được trích ly trong hạt cau. Kết quả nghiên cứu thu được mô hình mô tả tách chiết hợp chất polyphenol từ hạt cau bằng hàm mục tiêu Y = 80,64 + 3,10X1 - 1,27X3 - 6,00X1 2 - 3,66X2 2 - 5,07X3 2. Dựa vào hàm mục tiêu, kết quả hàm lượng polyphenol được thu nhận cao nhất là 80,72 mg GAE/g nguyên liệu, với các điều kiện tối ưu như sau: nồng độ dung môi ethanol 55%, tỷ lệ ngâm DM/NL (v/w) 35/1 và thời gian ngâm 2 giờ. Kết quả hàm lượng polyphenol của thực nghiệm không có sự khác biệt có ý nghĩa thống kê so với kết quả dự đoán của mô hình. Kết quả này là tiền đề cho quy trình thu nhận hợp chất polyphenol từ hạt cau ứng dụng kháng khuẩn trong nông nghiệp và y dược.Polyphenols are a group of natural compounds found in plants with antibacterial and antioxidant properties, offering significant potential applications in pharmaceutical and agricultural production. To extract and obtain a high content of polyphenolic compounds from areca nuts (Areca catechu L.), the study utilised a surface response methodology to assess the impact of various technical factors such as solvent concentration (X1), solvent-tomaterial ratio (v/w) (X2), and soaking time (hours) (X3) on the yield of polyphenolic compounds (Y) extracted from areca nuts. The study's results yielded a model describing the extraction of polyphenolic compounds from areca nuts using the objective function Y = 80.64 + 3.10X1 - 1.27X3 - 6.00X1 2 - 3.66X2 2 - 5.07X3 2. Based on the objective function, the highest polyphenol content obtained was 80.72 mg GAE/g of raw material under the following conditions: ethanol solvent concentration of 55%, solventto- material ratio (v/w) of 35/1, and soaking time of 2 hours. The experimental polyphenol content results showed no statistically significant differences compared to the modelpredicted outcomes. These results serve as a basis for the polyphenol extraction process from areca nuts with antibacterial applications in agriculture and medicine.

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