In vitro tests at the air-liquid interface (ALI) represent valuable alternatives to animal experiments to assess the acute toxicity of inhalable compounds. However, these methods still need to be characterized for the toxicity evaluation of complex mixtures such as combustion smokes. In this study, Alveolar type I or Alveolar type 2 cells in co-culture with macrophages were investigated as models for evaluating the acute toxicity of complex mixtures at the air-liquid interface. In that purpose, smokes/obscurants were generated from pyrotechnic devices of known toxic potentials in a 1.3 m