The authors investigated the systematic position of 11 dipterocarp species (Dipterocarpaceae) in provinces: Dong Nai, Dak Lak, Tay Ninh and Phu Tho based on analysis of matK region. DNA total was extracted from inbarks. The nucleotide sequence of matK was determined with 1056-1111bp, an average of 1073bp and used to analysis of phylogeny of 11 dipterocarp species (eight in Dipterocarpus genus, one in Hopea and one Vatica) using neighbor joining method. The analysis indicated low interspecific divergence among Dipterocarpus species, ranging from 0 to 0.9 percent. The high gene distance was found between Vatica/Dipterocarpus or HopealDipterocarpus. All Dipterocarpus species formed monophyletic clade with bootstrap value of 100 percent.