Establishment of the specimen collection for teaching is important, particularly in improving the quality of teaching. This study aims at species composition and development of collection of the snakes in Dong Thap province. The field research was conducted from 2008 to 2012. The research could develop of a list of 32 species of snakes belonging to 7 families, 21 genera distributed in Dong Thap province. Family Colubridae has 13 genera (61.9 percent of the total), family Elapidae has 3 (14.28 percent of the total), the rest have a (4.7 percent of the total). From specimens collected in the wild, the authors have made the specimens- exhibited in Laboratory of Zoology, faculty of Biology, Dong Thap University.