The species diversity of macromycoflora of the Phong Dien Nature Reserve - Thua Thien Hue province is very high. The origin of the macromycoflora of the Phong Dien Nature Reserve - Thua Thien Hue province are seven mycogeographycal areas: Cosmopolite, North hemisphere, Pantropic, Paleotropic, North temperate, East Asia and South China. In the study, the authors have identified 162 species belonging to genera,30 families and 18 orders of 2 phyla (Ascomycota and Basidiomycota). Among them, 21 species are new species to the macromycoflora of Vietnam. The most dominant taxa are: Basidiomycota Poriales, Coriolaceae, and Ganoderma. There are 24 edible mushrooms species, 14 medicinal species, 4 poisonous mushrooms species, 117 forestry wood saprophytic species, and 14 plant parasitic species. Two species are included in The Viet Nam Red Data Book (2007): Lentinus sajor-caju (Fr.) Fr. is an endangered species (EN) and Cookeina tricholoma (Mont.) Kuntze is a vulnerable (VU) species.