The giant mushroom Macrocybe gigantea (Massee) Pegler & Lodge, newly found in Lai Thieu, Thuan An, Binh Duong province, determined by conventional and molecular methods. Molecular method specified that used specimen has rDNA data at 99 percent similar with that of GenBank. The mycelial growth reached up to 6,8mm/d on modified PGA and 3,2mm on rice grain media, respectively. Fruitful cultivations have been done in Dalat with bags containing 2000g of rubber tree sawdust, supplemented with 10 percent rice, 5 percent corn bran, 1 percent CaCO3, 0,1 percent KNO3
60 +/- 5d for filling mycelia and 240d for incubation to form cluster primordia and numerous fruit bodies. The biological efficiency was about 40 percent.