Rubber seed oil was analyzed for physical properties and chemical components aiming to biodiesel synthesis process. Some properties such as density, pour point, flash point, acid number etc. were determined by the ASTM methods with the rubber seed oil before and after heat treatment at 120°C combined with settling and filtering process. The physical properties were also analyzed when the oil was stored in 6 months to estimate its potential usages and changes. The rubber seed oil was also characterized by GC-MS method to determine its the fatty acid composition. The results showed that there were a dominate percentage of unsaturated fatty acids supporting for very good cold properties of the oil and the products derived from it.