Acid-solubilized collagen (ASC) was extracted from basa fish skin (Pangasius hypophthalmus) by acetic acid and purified by dialysis, lyophilization and supercritical carbon dioxide (SC-CO2) extraction techniques. After 24h under stirring condition, more than 93 percent of salt was removed by dialysis. The efficiency of lipid extraction process reached 91.6 percent at the following conditions: pressure of 200 bar, CO2 flow rate of 10 g/min, temperature of 38°C, concentration of ethanol as co-solvent of 10 percent (v/v) and the retention time of 60 minutes. The obtained collagen has low impurities of lipid (0.33 percent), and ashes (0.29 percent). Moreover, extracted collagen has no smell, no color, high molecular weight, and is appropriate for cosmetic purposes, pharmaceutical and food applications.