Cố định Cellulase từ nấm Trichoderma sp. trên hạt composit tạo bởi Chitosan và canh lanh hoạt hóa

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Tác giả: Hữu Chí Đỗ, Hữu Nghị Đỗ, Mai Hương Lê, Thị Hồng Hà Trần, Thị Như Hằng Trần, Đình Giáp Vũ

Ngôn ngữ: vie

Ký hiệu phân loại: 589.95 [Unassigned]

Thông tin xuất bản: Khoa học và Công nghệ, 2014

Mô tả vật lý: 557-566

Bộ sưu tập: Metadata

ID: 503811

In the present study, the composite beads were successfully prepared using chitosan and activated kaolin, which had a diameter of approx. 4.5 mm and pore size of 10 - 50 um. Cellulase was purified from liquid-state cultivation of Trichoderma sp. by using ion-exchange (DEAEcellulose) and size-exclusion (Sephadex G 100) chromatography, and was afterward immobilized on the composite beads with the yield of 78.7 percent. The immobilization efficiency, stabilities of composite particle and immobilized enzyme as well as operational stability were evaluated. The pH - and thermal stabilities of immobilized enzyme were significantly higher than those of the free enzyme (remaining activity increased 35 percent as incubated at 60°C and 22 percent at pH 9.0). The immobilized enzyme has a reusability of at least 4 times. It remained more than 50 percent of its activity after 12 times of reuse.
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