Infection diseases cause of death for patients at the same number of patients died by traffic accidents, AIDS and breast cancer. When the bacterial cells have a chance to settle on the surface of graft matetials, they will change the phenotype and produce mucus to form a new form called biofilm to protect themselves against the immune system of the body and the effects of antibiotics. The tibrin gel has many usetul characteristics as biocompatibility, biodegradation in the human body environment, adhesion and spongy structure for development of antibiotic delivery system to prevent the formation of biofilm. In this study, human plasma was collected form human peripheral blood. After that the authors created a tibrin gel directly from human plasma by supplement ion Ca2+, then gel was evaluated gel formed by scanning electron microscope (SEM) and degradation in vitro. Fibrin gel containing antibiotics was assessed through ability to deliver antibiotics in vitro, effect on biofilm bacteria, and poison for human tibroblast cells in vitro. Of the investigated concentration of CaCl2 applied for the tibrin gel formation, the gel created by higher concentration of CaCl2 was degraded in vitro lower than the gel generated by lower concentration of CaCl2. Most important, tibrin gels containing antibiotic were not toxic for human tibroblast cells in vitro and inhibited the growth of biofilm bacteria in vitro. In Viet Nam, this issue is still very new
virtually no scientitic studies published concerning. This research as the tirst step for the development of antibiotic delivery for effective treatments for infection diseases.