In this study, the authors accessed the effect of human leukemia inhibitory factor (hLIF) on invitrp development of bovine embryo in preimplantation stages. The number of bovine embryos at different stages was determined to evaluate the development of embryos and the cell number of bovine blastocyst embryos ana inner cell mass were counted to estimate the quality of blastocyst embryos. The results showed that hLIF did not effect on bovine embryo cleavage from zygote stage to 2-cell stage. 10 exponent 3 IU/ml LIF supplementation in bovine embryo culture medium supported to embryo division from 2-cell stage to 4-cell stage (31,085). The ratio of embryo division from 2-cell stage to 4-cell stage of control group and 10 exponent 2 IU/ml LIF supplemented group was lower than that of 10 exponent 3 IU/ml LIF supplemented group. In 10 exponent 3 IU/ml LIF supplemented group, the ratio of bovine embryo in 8-cell stage (28,71 percent) and morula stage (18,24 percent) was higher than those of control group and 10 exponent 2 IU/ml LIF supplemented group. Thus, LIF could rescue to bovine embryo block from 8-cell stage to 16-cell stage. The ratio of blatocyst embryo in 10 exponent 3 IU/ml LIF supplemented group was higher than control group and 10 exponent 2 IU/ml LIF supplemented group. The mature oocytes, 2 and 4-cell stage embryos, 8 and 16-cell stage embryos, and blastocyst embryo expressed LIF transcription. The bovine blastocyst embryo quality in 10 exponent 3 IU/ml LIF supplemented was higher than control group and 10 exponent 2 IU/ml LIF supplemented group by accessing cell number in inner cell mass and blastocyst embryo. These results showed that LIF could positively support to bovine in vitro development in preimplantation stage.