Gamma irradiation technique combined with tissue culture and in vitro flowering was applied in this study. The results showed that the frequences of variation in plant regeneration from irradiated leaf samples were: 0.67 percent (with 30 Gy dose) and 0.72 percent (with 40 Gy dose) in MV 1 generation
the frequences of variation in irradiated plantlet samples were: 1.05 percent (with 30 Gy dose) and 1.15 percent (with 40 Gy dose) in MV4 generation, the frequences of mosaic were 0.25 percent and 0.08 percent in MV 3 and MV 4 generation, respectively. A total of 16 mutants were selected based on phenotypic variations going thrmlgh screening processes of tissue culture and in vitro flowering. Three promising mutant lines (G40TP1, G40TP2, G30TL1) presented a high genetic stability through generations cultivated in both in vitro and ex vitro conditions when being compared with the controls. These mutant lines G40TP1, G40TP2, G30TL1 had a high potential to become new cultivars. This paper showed that the application of in vitro flowering technique for mutation breeding of Torellia (Torenia fournieri L.) is a significant complementary and effective model for selecting mutants produced by irradiation.