Phân lập gen Os NAC10 liên quan tới tính chống chịu hạn từ giống lúa Indica

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Tác giả: Duy Phương Nguyễn, Thu Hằng Phạm, Xuân Hội Phạm

Ngôn ngữ: vie

Ký hiệu phân loại: 571.962 Physiology and related subjects

Thông tin xuất bản: Công nghệ sinh học, 2014

Mô tả vật lý: 319-326

Bộ sưu tập: Metadata

ID: 504228

Plants response to adverse environment by initating a series of signaling processes including activation of transcription factors that can regulate expression of various stress-responsive and adaptive genes. NAC family (NAM, ATAF1/2, CUC2), which is the largest plant transcription factor family, plays a important role in development and stress responses in plant. OsNAC10, is expressed predominantly in roots and panicles and induced by drought, high salinity, and abscisic acid. Overexpression of OsNAC10 in rice increased the plant tolerance to drought, high salinity, and low temperature at the vegetative stage. More importantly, the OsNAC10 plants showed significantly enhanced drought tolerance at the reproductive stage. In this study, the authors isolated OsNAC10 gene from drought-treated Indica rice cDNA. As a result, the length of Indica rice OsNAC10 cDNA is 1183 nucleotides, contains a 516 bp-ORF encoding for OsNAC10 protein. Amino acid sequence alignment of Indica OsNAC10 protein and homologs GeneBank-registed OsNAC10 (NM_001072159.1 and NM_001072566.2) shows that they had striking homology (92,03 percent and 99,17 percent, respectively). Analysis of its deduced amino acid sequence indicated that this protein contains four conserve domains and one putative nuclear localization signal at N-terminallike all well-known NAC proteins.
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