In this study, the effects of different light conditions on the shoot multiplication
the growth and development as well as the contents of chlorophyll a and b of Chrysanthemum cultured in vitro were investigated. Chrysanthemum shoots were cultured under different light conditions, specifically red LED, blue LED, yellow LED, green LED, white LED, red and blue LED in combination (R:B = 10:90, 20:80, 30:70, 40:60, 50:50, 60:40, 70:30, 80:20, 90: 10, respectively). After 4 weeks of culture, the results indicated the best light condition for shoot multiplication was 50 percent red LED plus 50 percent blue LED. The combination of 70 percent red LED and 30 percent blue LED gave the best results in term of fresh weight, dry weight, length and width of leaf (1.25 g
0.07 g
1.72 cm
1.60 cm
respectively). In addition, the contents of chlorophyll a, b depended on different light conditions. The highest contents of chlorophyll a (29.20 ug/cm2) and chlorophyll b (14.57 ug/cm2) were obtained under 70 percent red LED and 30 percent blue LED. Thus, the best shoot multiplication was obtained under 50 percent red LED plus 50 percent blue LED
however, the combination of 70 percent red LED and 30 percent blue LED were optimal for the growth and development as well as the contents of chlorophyll a and b of Chrysanthemum.