Inflammatory responses revealed through HIV infection of microglia-containing cerebral organoids.

• Tác giả: Lena Al-Harthi, Michelle K Ash, Stephanie Gambut, Ramon Lorenzo-Redondo, João I Mamede, Charia McKee, Srinivas D Narasipura, Anjelica F Reyes, Jeffrey R Schneider, Tanner Shull, James L A Szczerkowski, Janet P Zayas
• Ngôn ngữ: eng
• Ký hiệu phân loại: 920.71 Men
• Thông tin xuất bản: England : Journal of neuroinflammation , 2025
• Bộ sưu tập: NCBI
• ID: 50425

Tóm tắt

Cerebral organoids (COs) are valuable tools for studying the intricate interplay between glial cells and neurons in brain development and disease, including HIV-associated neuroinflammation. We developed a novel approach to generate microglia containing COs (CO-iMs) by co-culturing hematopoietic progenitors and inducing pluripotent stem cells. This approach allowed for the differentiation of microglia within the organoids concomitantly with the neuronal progenitors. Compared with conventional COs, CO-iMs were more efficient at generating CD45