Sex identification in avian species is one of the key points of avian breeding and evolutionary studies, Many avian species are considered sexually monomorphic. In monomorphic bird species, especially in young birds, it's is difficult to identify birds' sex based on only their external ,morphology. Through, vent sexing, laparoscopy, steroid sexing and karyotyping are methods for sex of birds. The sex of an individual is established from the genes located on sex chromosomes. Per one somatic cell, female birds have one copy of both Z and W chromosome, and male birds have two copies of Z avian sex chromosomes. The primer pairs using are 2550F, 2718R (Fridolfsson va Ellegren, 1999) amplifying sex specific CHD1 gene of both Z and W chromosomes. There are five samples (three tissue samples lid two incubated egg samples) for sex identification. The single band observed on 1.5 percent agarose gel llectrophoresis of five samples. DNA sequence of NDB2 sample belong to CHD1W of female. Thus, the lie samples belong to females.