The techniques of the seed production of nemo anemonefishes have been studied successfully with the survival rates of one-month-old fish reaching approximately 35 percent. The studied results showed that: To quickly achieve broodstock maturation and reproduction in captivity, the pairs should be acclimatized at the seawater temperatures range from 25°C - 30°C without the presence of favourite anemone host and the most suitable culture volume was 300 litres. Newly fish from 1 to 5 days old were fed on rotifers (Brachionus plicatilis) with density of 5-7 inds/ml combining microalgae (Nannochloropsis oculata). When the fish reached about 7 days old, they were fed on Artemia until the fish reached. commercial size. After month-old, the fish were fed on Artemia combining with artificial food. The cultured nemo anemone fishes can adapt at the salinity from 10-40 thounsandth. However, the best salinity for growth of the juveniles ranged from 30-35 thounsandth.