To determine the materials sources for breeding groundnut varieties resistant to bacterial wilt, artificial inoculation in combination with molecular markers were used. The result on evaluation of resistance ability to bacterial wilt of 63 lineslvarieties of peanut germ plasm by artificial inoculation showed that. there were 3.2 percent of highly resistant. 11.1 percent resistant, 34.9 percent moderate resistant. 30.1 percent moderate susceptible, 15.9 percent susceptible and 4.8 percent highly susceptible lines/varieties. Using three SSR markers: pPGPSeq3F5, 7G2 and GA161 to determine the resistance to bacterial wilt of 63 lines/varieties of peanut germplasm, the result indicated that 26 lines/varieties (0713.24.1, ICGV6022, BW62, CG 38, L 17, ICGV01239, 0401.57.1, line 14, ICGV01232, ICGV01238, L16, VAG 03.2, line 2, ICGV01241, ICG 5051, BW15, BWP-1, BWP-3, BWP-5, BWP-6, BWP-7, BWP-8, BWP-11, BWP-12, BWP-13 and Gie Nho Quan) were resistant to bacterial wilt on artificial inoculation but they also showed resistant ability through selection by using three SSR makers associated with resistance traits. The 26 lines/varieties were determined to be resistant to bacterial wilt and could be used as materials for breeding programs.