According to World Health Organization (WHO), there are approximate 8 millions people suffering from hepatitis B or hepatitis C in Vietnam currently. Chronic hepatitis B is the main reason causing liver diseases in Vietnam such as cirrhosis and liver cancer. Along side with ordinary drug treatment therapies, transplanting stem cells orientating liver cells is a novel method which is being investigated for further applications. In all kinds of stem cells, umbilical cord stem cell is a various, abundant and available source which does not violate biomedical ethics. In this study, to initially differentiate umbilical cord mesenchymal stem cells. into hepatic cells, the authors transfected coding sequence of the HNF-4a gene - a transcription factor for more than 100 genes in liver cell into umbilical cord stem cells through expression vector pCMV-EGFP. Moreover, the expression at molecular level of specific molecular markers for mesenchymal stem cell such as CD86, CD90, CD105, CD73... were evaluated. Results after transfection displayed HNF-4a and all mesenchymal stem cell markers presented in stem cell based on reverse transcription-PCR method. The achievement of this study has established a new research trend which possesses big significances in treament liver-related diseases for patients.