Elicitors are known as compounds that when introduced into a living organism signal the activation or synthesis of another compound. In vitro plant materials are good sources for the production of secondary metabolite and elicitation can be used as one of the effective tools in order to improve the synthesis of these compounds. In this study, abscisic acid, jasmonic acid and salicylic acid were added to the culture medium in order to examine effects of these elicitors on growth and ginsenoside production of secondary roots of Panax vietnamensis. After 4 weeks, results indicated that elicitors strongly inhibited secondary roots growth. However, these elicitors made a contribution to ginsenoside production improvement. Thin layer chromatography (TLC) and High-performance liquid chromatography (HPLC) analyses of the roots cultured on SH medium supplemented with 5 mg/l IBA, 30 g/l sucrose, 8 g/l agar and one of these elicitors showed the presence of ginsenoside MR2, RbI and RgI at different contents. The highest contents of MR2 (2.826445 percent) and total saponin (3.42449 percent) in secondary roots were recorded when explants were cultured on medium containing 200 ul/l jasmonic acid.