Lily is one of the most favourite flowers which have high value producing, nice color, fragance and preffered by consumers. In Vietnam, lilies have been planted since many years ago in Dalat, Lam Dong province and get increased in quantity and scale, recently. Lilies have been also grown in the North of Vietnam since 200 I with small scale . Vietnam and many other countries have not produced lily bulbs, so they have to import from Netherland at quanity of about 60 million lily bulbs for production. Breeding lily varieties with beautiful color, fragrance and suitable for the climate conditions in Vietnam is nessesary trend for the breeders. Nowaday, plants breeding by using molecular markers is getting more and more applicable in the world and in Vietnam. However, there are few studies using ISSR markers to breed lily varieties. This study presents the evaluation some lily hybrids combinations by using 14 ISSR markers. The results showed that the lines 134 (L5) and SL11(LLK11) have been identified as the hybrids of the respective combination of Bel (Lilium Oriental Trumpet hybrid lily 'Be/ladonnd) x Sor (Lilium Oriental hybrid lily 'Sorbonne') and Sor (Lilium Oriental hybrid lily 'Sorbonne') x L (Lilium longiflorum). Of the combinations of F (Liliumformolongo) x L(Lilium longiflorum), 15(LKS) line is actually identified as a hybrid of the combination, while 14 line is evaluated to have the same genotype as F line. Therefore, it is concluded that 14 developed from an asexual embryo in the F line.