Globo H molecules, which was first isolated from breast cancer MCF-7 cells, are glycan with chemical structure as hexasaccharide. Globo H apprears as an important cancer antigen. Based on its ability to cause immune response, Globo H becomes a promissing target for anti-cancer vaccine researches and development. In order to identify the potential immunological response against tumors of Globo H, in the study, those molecules were firstly conjugated with Keyhole Limpet Hemocyanin (KLH). As the next step, the conjugated Globo H-KLH complex were then vaccinated to albino BALB/c mice at different concentrations which were I, 2 and 4 !tg/mouse/times by exactly following the immunized protocol of Liddell & Cryer (1991) with minor modification. As soon as the immunized process had been completed, the vaccinated mice were promptly tumorized with Lewis Lung Carcinoma (LLC) cells for cancer developed model. By the time of ending experiment at the day 35, all immunized mice with Globo H-KLH at different concentrations survived while the negative control group had resulted with 2 dead mice (account for 33.33 percent). The results also exhibited that mice which were immunized with 4 !tg/mouse/times of Globo H-KLH antigen could prevent tumor growth up to 66.67 percent, together with 100 percent of mice survival. The results again indicated the stabilization ofhaematological parameters of immunized mice in comparision with those of the controls. Although showing as the initial results, the study proves the positive and promising vaccinated activities of Globo H in terms of immunotherapy for cancer prevention in the future.