The experiment was conducted for Acacia hybrid clone KL2. Explants were cleaned by soap and disinfected by 75° alcohol then disinfected by HgCL2 chemical with concentrations of 0.1 percent and 0.5 percent. Each concentration was disinfected by 4 times: two, five, eight, and ten minutes. The results were assessed after 4 weeks including the sample rate of bacterial yeast infection, rate of death, rate of clean samples without budding and rate of clean samples with budding. These showed the concentration of HgCL2 0.5 percent at eight minutes giving the best effect with the rate of clean sample and budding of 17.3 percent, and this concentration was statistically significant differences fiom others with a=0.05.