Điều chế nhanh trong phòng thí nghiệm các enzym của các vật liệu tổng hợp hydro sinh học tương thích từ chất dẫn xuất chitosan và các hạt nano phốt phát canxi pha kép cho việc tái sinh xương

• Ngôn ngữ: vie
• Ký hiệu phân loại: 620.5 Nanotechnology
• Thông tin xuất bản: Nanoscience and Nanotechnology, 2014
• Mô tả vật lý: 45296
• Bộ sưu tập: Metadata
• ID: 506382

Tóm tắt

Injectable chitosan-based hydrogels have been widely studied toward biomedical applications because of their potential performance in drug/cell delivery and tissue regeneration. In this study the authors introduce tetronic-grafted chitosan containing tyramine moieties which have been utilized for in situ enzyme-mediated hydrogel preparation. The hydrogel can be used to load nanoparticles (NPs) of biphasic calcium phosphate (BCP). mixture of hydroxyapatite (HAp) and tricalcium phosphate (TCP), forming injectable biocomposites. The grafted copolymers were well-characterized by 1H NMR. BCP nanoparticles were prepared by precipitation method under ultrasonic irradiation and then characterized by using x-ray powder diffraction (XRD) and scanning electron microscopy (SEM). The suspension of the copolymer and BCP nanoparticles rapidly formed hydrogel biocomposite within a few seconds of the presence of horseradish peroxidase (HRP) and hydrogen peroxide (H2O2). The compressive stress failure of the wet hydrogel was at 591 + or - 20 KPa with the composite 10 wt percent BCP loading. In vitro study using mesenchymal stem cells showed that the composites were biocompatible and cells are well-attached on the surfaces.