Khai thác và đặc tính hóa của Exopolysaccharides từ Azotobacter Chroococum AT19

• Ngôn ngữ: vie
• Ký hiệu phân loại: 589.95 [Unassigned]
• Thông tin xuất bản: Khoa học và Công nghệ các trường đại học kỹ thuật, 2014
• Mô tả vật lý: 45295
• Bộ sưu tập: Metadata
• ID: 506524

Tóm tắt

Azotobacter chroococcum is an amylolytic, nitrogen-fixing bacterium with an extremely high rate of respiration. It is quite common and believed to contribute significantly to non-symbiotic nitrogen fixation in soil. Beside that it produces appreciable amounts of extracellular polysaccharide (EPS). The EPS is used in the food, pharmaceutical and biomedical fields. An A. chroococcum strain was selected by its highest extracellular polysaccharide (EPS) production ability compare to other isolates from the soil. Produced exopolysaccharide by A. chroococcum strain AT19 was obtained 6.2 g/I from fermentation done at 30°C for 4 days with shaking at 180 rpm. The exopolysaccharide was extracted by ethanol. The HPLC analysis of the EPS hydrolysate by TFA showed that the EPS of A. chroococcum strain AT19 was composed of 16.61 percent of glucose and 4.42 percent galactose. This EPS may find possible application as a polymer for environmental bioremediation and biotechnological processes.