The 1,4-Dioxane (1,4-D) is a carcinogenic compound. The treatment of 1,4-D in wastewater is still a major challenge ahead. Microbial degradation of 1,4-D would be an attractive bioremediation approach. In this study, the dextrose and tetrahydrofuran (THF) were added to examine the biodegradation process of 1,4-D. As a result, the bacterial consorlium enriched from industrial sludge was capable of degrading 1,4-0 effectively. The enriched culture was able to degrade 1,4-D as sole carbon and energy source. The added dextrose was consumed together with 1,4-D by enriched culture. The addition of dextrose did not influence on 1,4-D biodegradation as its specific biodegradation rate was found to be a little change. Whereas, the addition of THF was proved as a competitive inhibition of 1,4-D since it negatively effect on specific degradation rate. The present work on 1,4-D could add more information in the biodegradation and the role of additional carbon sources in the biodegradation process.