Biến đổi hóa học của microsieves nitride silicon để nắm bắt sự lưu hành của các tế bào khối u MCF-7 của bệnh nhân ung thư vú

• Ngôn ngữ: vie
• Ký hiệu phân loại: 620.5 Nanotechnology
• Thông tin xuất bản: Nanoscience and nanotechnology, 2015
• Mô tả vật lý: 45298
• Bộ sưu tập: Metadata
• ID: 509186

Tóm tắt

Chemical modification of silicon nitride (SiN) microsieves with glutaraldehyde and 3-glycidoxypropyldimethylethoxysilane (GapS) for bio-coupling with an antibody to capture MCF-7 circulating tumor cells of breast cancer is reported. In this research, the antibody monoclonal anti-cytokerantin-FITC with fluorescein isothiocyanate label was used due to its good selectivity to MCF-7 circulating tumor cells of breast cancer. Modification efficiency was determined by the variation of contact angle. The increase in contact angle of the microsieves treated with glutaraldehyde and GapS indicated that the micro sieve surface changed from hydrophilic to hydrophobic. These results confirmed the successful immobilization of glutaraldehyde and GapS onto SiN microsieves. Antibody binding effect was evaluated by fluorescence microscopy. Fluorescent images exhibited that GapS was more effective than the glutaraldehyde treatment The GaPS-treated microsieves were then used for capture of MCF-7 cells in phosphate buffered saline (PBS). The fluorescent images proved that the surface modification of SiN microsieves with GapS helped to increase the efficiency of MCF-7 capture.