BACKGROUND: Cystic neutrophilic granulomatous mastitis (CNGM) poses a significant threat to the physical and mental health of women due to its increasing incidence, complex clinical manifestations. Developing an appropriate animal model will help further study the pathogenesis of CNGM. METHODS: Seventy-two rats were randomly assigned to seven groups: group A (n=12, tissue suspension 0.2mL), group B (n=12, 1×10^8 CFU/mL RESULTS: Rats in groups A, D, and E exhibited breast masses with erythema, with some showing ulceration, and granulomatous structures in pathological. Lipid vacuoles and Gram-positive rods observed in groups D and E. Pus cultures from groups D and E showed growth of CONCLUSION: Components of pathological tissues from granulomatous mastitis patient combined with