The global agricultural sector has persistently grappled with the effective handling of bovine waste. This research repurposes bovine waste into a cost-effective and sustainable fiber, which is subsequently treated with dendritic fibrous nanosilica (DFNS) to produce CDF/DFNS. This is then integrated into cement-based composites. This study aimed to introduce CDF/DFNS to enhance the operational efficiency and longevity of Portland cement with notable sulfate resistance. Concurrently, CDF/DFNS was incorporated into cement mortar, and an examination of the aggregation of CDF/DFNS, along with the aquation procedure and microstructure of the mortar, was conducted. The investigation indicated that incorporating CDF/DFNS enhanced the toughness, tangible attributes, and imperishability of the cement specimens. The integration of CDF/DFNS resulted in a reduction in both the parameters related to the movement of chloride ions and the volume of voids in a material. Furthermore, the ability of cement mortar to resist compression forces was improved with the presence of CDF/DFNS as opposed to control samples. These improvements in strength and imperishability suggested that CDF/DFNS holds significant potential in reducing CO