Pre-Steady-State Kinetic Studies of Nucleotide Incorporation into a Single-Nucleotide Gapped DNA Substrate Catalyzed by Human DNA Polymerase β.

• Tác giả: Daniel Betancourt, Turner W Seay, Zucai Suo, Nikita Zalenski
• Ngôn ngữ: eng
• Ký hiệu phân loại: 001.4 Research; statistical methods
• Thông tin xuất bản: United States : Biochemistry , 2025
• Bộ sưu tập: NCBI
• ID: 52080

Tóm tắt

DNA polymerase β (Polβ) is a key enzyme in DNA base excision repair (BER). Despite extensive research, several microscopic rate constants within the kinetic mechanism of nucleotide incorporation into single-nucleotide gapped DNA by Polβ have not been determined and the identity of the rate-limiting step remains controversial. Here, we employed pre-steady-state kinetic methods and determined the rate constants for correct dNTP association (