Chọn tạo các dòng ngô kháng bệnh mốc hồng bằng chỉ thị phân tử SSR

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Tác giả: Thị Hương Hồ, Thị Bích Thủy Lê, Thị Thùy Linh Ngô, Văn Cảnh Nguyễn, Huy Minh Vương

Ngôn ngữ: vie

Ký hiệu phân loại: 571.962 Physiology and related subjects

Thông tin xuất bản: Khoa học & Công nghệ Việt Nam, 2018

Mô tả vật lý: 60-64

Bộ sưu tập: Metadata

ID: 534629

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Bệnh mốc hồng là một trong những bệnh gây tổn thất lớn cho sản xuất ngô trên thế giới nói chung, Việt Nam nói riêng. Kết quả chọn giống kháng bệnh theo phương pháp truyền thống còn hạn chế do hiệu quả chuyên các gen kháng bệnh vào con lai còn khó khăn và tốn nhiều thời gian. Vì vậy, việc ứng dụng chỉ thị phần tử trong chọn tạo giống ngô kháng bệnh mốc hồng là phương pháp khả thi để kiểm soát dịch bệnh. Trong các nghiên cứu trước đây, qua phân tích SSR và đánh giá khả năng kháng bệnh của tập đoàn ngô đã xác định được sự liên kết của 6 chỉ thị SSR (Umc1025, Dupssr34, Nc030, SSR93, Umc1489 và Umc1511) với tính trạng kháng bệnh mốc hồng. Trong bài báo này, các tác giả sử dụng 6 chỉ thị để đánh giá trên quần thể phân ly F5 và quần thể lại ngược BC5 để chọn các dòng ngô triển vọng kháng bệnh mốc hồng. Kết quả chọn được 11 dòng có chỉ thị liên kế với tính kháng bệnh mốc hồng, trong đó 3 dòng có 1 chỉ thị, 5 dòng có 2 chỉ thị, 1 dòng có 3 chỉ thị và 2 dòng có 4 chỉ thị liên kết
8 dòng (F5.5, F5.12, F5.18, F5.22, BC5.8, BC5.9, BC5.21 và BC5.22) có ít nhất 2 chỉ thị liên kết với tính kháng bệnh mốc hồng được lựa chọn cho chương trình lai tạo giống
các tổ hợp lai (THL) THL5, THL25, THL6 và THL12 có ít nhất 3 chỉ thị liên kết được lựa chọn cho các thử nghiệm trong sản xuất.Pink mold disease is one of the diseases that cause great losses to maize production in the world in general and in Vietnam in particular. The results of traditional disease resistance selection are still limited due to the difficulty and time-consuming transfer of disease-resistant genes into crosses. Therefore, the application of the indicator in the breeding of maize resistant to pink mold is a feasible method to control the disease. In the previous studies, through SSR analysis and disease resistance assessment of the corn corporation, the association of 6 SSR indicators (Umc1025, Dupssr34, Nc030, SSR93, Umc1489 and Umc1511) has been identified. resistance to pink mold. In this paper, the authors used 6 indicators to evaluate on F5 dissociated populations and BC5 inverse populations to select promising maize resistant maize lines. The result was to select 11 lines with adjacent indications with pink mold resistance, of which 3 lines had 1 indicator, 5 lines had 2 indicators, 1 line had 3 directives and 2 lines had 4 associated markers
8 lines (F5.5, F5.12, F5.18, F5.22, BC5.8, BC5.9, BC5.21 and BC5.22) have at least 2 indicators associated with resistance to pink mold. selections for breeding programs
Hybrid combinations (THL) THL5, THL25, THL6 and THL12 have at least 3 link indicators selected for production trials.

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