The article presents the first study of the most complete composition of natural enemies of Dien grapefruit tree. Fluctuation in specie component of predators (natural enemies) of Dien grapefruit tree insect pests differed over the months of this investigation with Coleoptera species being most prevalent with 7 species, accounting for 38.89 percent of the total species and Hymenoptera species numbering 5, accounting for 27.78 percent. The numbers of species of natural enemies of Dien grapefruit tree insect pests differed over the months of this survey: Species composition of natural enemies of Dien grapefruit tree insect pests was highest at 18 species in September when 100 percent of the natural enemies of Dien grapefruit tree insect pest species of the list were found. In June 2013, and in January 2014, 17 species were found, in July 16 species were found, in June 15 species were found, in December 14 species were found, in October 13 species were found and in November only 11 species were found.