Chiral covalent organic frameworks (CCOFs) are emerging porous materials with tunable chiral structures, abundant pores, and high surface areas, gaining significant attentions in separation, sensing, and asymmetric catalysis. This review summarizes the synthesis methods of CCOFs and their applications in chiral recognition. It discusses the advantages and limitations of three synthesis strategies, including chiral post-modification, direct synthesis, and chiral-induced synthesis. The review also highlights the potential of CCOFs in chiral separation, sensing, and asymmetric catalysis for efficient purification, detection, and synthesis of chiral molecules. Challenges and future directions for the preparation and application of CCOFs are also addressed, aiming to guide further research and practical applications.