Phân lập và định danh Leptospira từ nước tiểu chó nghi mắc bệnh Leptospirosis ở Thành phố Cần Thơ=Isolation and identifcation of Leptospira from the urine of dogs with suspected leptospirosis in Can Tho city

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Tác giả: Mười Nguyễn Thị Bé, Trần Đình Từ, Việt Thu Hồ Thị

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học kỹ thuật Thú y, 2018

Mô tả vật lý: tr.42

Bộ sưu tập: Báo, Tạp chí

ID: 541851

Thêm vào giỏ

leptospirosis is a popularly zoonotic disease of the worldwide. The sero-prevalence of leptospirosiswas reported in the domestic dogs in the Mekong Delta. Recently, the dogs suspecting leptospirosiswith the clinical syndromes, such as: jaundice, mucosal hemorrhage, oculo-nasal discharge, abdominalpain and renal dysfunction were recorded in the veterinary clinics in the investigating area. The objectiveof this study was to detect the Leptospira species causing leptospirosis in the dogs. One hundredand eleven (111) urine samples were collected from the suspected dogs to detect the presence ofleptospira by a dark-field microscopy
leptospira from the positive samples was inoculated in the EMJHmedium, then Leptospira species were identified by PCR assay, and 16S rRNA gene sequence.The results of dark-field microscopic examination showed that leptospira was present in 63 out of111 (56.75%) urine samples, however leptospira was cultured successfully on EMJH medium fromonly 3 out of 63 positive urine samples, accounting for 4.76%. The results of analysing leptospira genesequences revealed two species, i.e. Leptospira interrogans and Leptospira fainei presenting in theurine of the investigated dogs, of which L. interrogans belonged to the mainly pathogenic group and L.fainei belonged to the associated pathogenic group. This is the first successful study on isolation andidentification of pathogenic Leptospira from the urine of the dogs suspecting leptospirosis in Viet Namleptospirosis là một bệnh truyền nhiễm chung của nhiều loài động vật và người, hiện diện phổ biếnkhắp thế giới. Sự lưu hành huyết thanh học của Leptospira được ghi nhận trên chó nuôi ở khu vực đồngbằng sông Cửu Long (ĐBSCL). Gần đây, xuất hiện những trường hợp chó nghi mắc leptospirosis vớitriệu chứng vàng da, xuất huyết niêm mạc, chảy nước mắt, đau cơ và rối loạn thận-niệu… đã được đưađến khám và điều trị tại các bệnh xá thú y trong khu vực. Mục tiêu của nghiên cứu này là phát hiện xoắnkhuẩn Leptospira gây bệnh trên chó. Một trăm mười một (111) mẫu nước tiểu chó có hiệu giá MAT≥1:400đã được thu thập để phát hiện sự hiện diện của xoắn khuẩn bằng phương pháp quan sát dưới kính hiển vinền đen. Xoắn khuẩn ở những mẫu nước tiểu dương tính được nuôi cấy tiếp trên môi trường EMJH, giámđịnh xoắn khuẩn bằng PCR và giải trình tự gen 16S rRNA để định danh loài.Kết quả quan sát bằng kính hiển vi nền đen đã phát hiện xoắn khuẩn hiện diện trong 63/111 (56,75%)mẫu nước tiểu, nhưng chỉ nuôi cấy Leptospira thành công trên môi trường EMJH từ 3 trong số 63 mẫunước tiểu này (chiếm tỷ lệ 4,76%). Kết quả giám định phân tử xoắn khuẩn đã phát hiện Leptospirainterrogans trong hai mẫu nước tiểu của chó có biểu hiện nhiễm bệnh lâm sàng và Leptospira fainei hiệndiện trong một mẫu nước tiểu của chó chưa biểu hiện bệnh, trong đó L. interrogans thuộc nhóm xoắnkhuẩn gây bệnh trực tiếp và L. fainei thuộc nhóm xoắn khuẩn gây bệnh cơ hội. Đây là kết quả phân lậpvà định danh xoắn khuẩn Leptospira gây bệnh từ nước tiểu chó đầu tiên ở Việt Nam.

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