GWAS-based population genetic analysis identifies bZIP29 as a heterotic gene in maize.

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Tác giả: Xuemei Du, Junjie Fu, Riliang Gu, Yong Jiang, Lin Li, Chengyong Liang, Yangyang Liu, Jiawen Lu, Yuting Ma, Xinxin Miao, Jochen C Reif, Renate H Schmidt, Jianhua Wang, Pingxi Wang, Xiaoli Wang, Yongrui Wu, Yuxin Xie, Zhenxiang Xu, Tao Yang, Hongwei Zhang, Jie Zhang, Sihan Zhen, Nannan Zheng

Ngôn ngữ: eng

Ký hiệu phân loại: 004.21 Systems analysis and design

Thông tin xuất bản: China : Plant communications , 2025

Mô tả vật lý:

Bộ sưu tập: NCBI

ID: 542855

Understanding the role of heterotic genes in contributing to heterosis is essential for advancing hybrid breeding. We analyzed plant height (PH), ear height (EH), and transcriptomic data from a maize hybrid population. GWAS identified that dominance effects of QTL play a significant role in hybrid traits and mid-parent heterosis. By integrating GWAS, expression GWAS (eGWAS) analysis, and module eGWAS, six candidate heterotic genes underlying six QTL were prioritized, including one QTL spanned the bZIP29 gene. In the hybrid population, bZIP29 exhibited additive expression and dominance effects for both traits and mid-parent heterosis, with its favorable allele correlating positively with PH and EH. bZIP29 demonstrated dominance or over-dominance patterns in the hybrids derived from crosses between transgenic and wildtype lines, contingent upon its expression. tsCUT&Tag assay revealed that bZIP29 protein bound directly to a gene regulated by its associated eQTL and six genes within expression modules governed by their meQTL. Regulatory networks involving bZIP29 were more extensive in hybrid sub-populations compared to the parental population. This study offers insights into key heterotic genes and networks underpinning the robust growth of hybrid maize.
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