Microfluidic vessel-on-chip platform for investigation of cellular defects in venous malformations and responses to various shear stress and flow conditions.

• Tác giả: Mohammadhassan Ansarizadeh, Laknee De Silva, Lauri Eklund, Ryan Hicks, Pauliina Junttila, Jere Kettunen, Bojana Lazovic, Hoang-Tuan Nguyen, Siiri-Liisa Rissanen, Prateek Singh, Ragul Sivakumar
• Ngôn ngữ: eng
• Thông tin xuất bản: England : Lab on a chip , 2025
• Bộ sưu tập: NCBI
• ID: 54419

Tóm tắt

A novel microfluidic platform was designed to study the cellular architecture of endothelial cells (ECs) in an environment replicating the 3D organization and flow of blood vessels. In particular, the platform was constructed to investigate EC defects in slow-flow venous malformations (VMs) under varying shear stress and flow conditions. The platform featured a standard microtiter plate footprint containing 32 microfluidic units capable of replicating wall shear stress (WSS) in normal veins and enabling precise control of shear stress and flow directionality without the need for complex pumping systems. Using genetically engineered human umbilical vein endothelial cells (HUVECs) and induced pluripotent stem cell (iPSC)-derived ECs (iECs) to express the recurrent