Phát triển dòng vi khuẩn biểu hiện độc tố đường ruột tái tổ hợp của vi khuẩn Enterotoxigenic escherichia coli gây tiêu chảy ở lợn con=Development of enterotoxin gene expression bacteria of enterotoxigenic Escherichia coli caused diarrheal disease in piglets

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Tác giả: Hải Lê Đình, Vũ Khắc Hùng, Thìn Võ Thành, Tuấn Đặng Văn

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học kỹ thuật Thú y, 2018

Mô tả vật lý: tr.43

Bộ sưu tập: Metadata

ID: 545160

Thêm vào giỏ

Mục tiêu của nghiên cứu là tạo được chủng vi khuẩn có khả năng biểu hiện độc tố đường ruột táitổ hợp. Trong nghiên cứu này, chuỗi gen mã hóa đồng thời ba loại độc tố đường ruột của vi khuẩn E.coli được khuếch đại và gắn vào vector pET 24a(+) và pET32a(+) và biến nạp thành công vào tế bàobiểu hiện E. coli BL21. Kết quả kiểm tra tạo dòng bằng phương pháp PCR và giải trình tự cho thấy, cácchủng vi khuẩn BL21 mang chuỗi gen tái tổ hợp có trình tự nucleotide phù hơp so với trình tự nucleotidecủa gen mã hóa các loại độc tố STa, STb và LT trên ngân hàng gen. Các chủng vi khuẩn mang chuỗi gentái tổ hợp có thể biểu hiện protein mong muốn trong môi trường LB broth với chất cảm ứng là IPTG.The objective of this study was to create recombinant enterotoxin gene expression cells. Thegenetic fusion of enterotoxin gene was amplified and attached into 24a(+) and pET32a(+) vectorand then transfored to BL21 successfully. PCR amplification and nucleotide sequence were used fordetermination of genetic fusion of enterotoxin gene in BL21 strain. The recombinant proteins wereevaluated by SDS-PAGE and the immunoreactivity was characterized by Western blotting. Thestudied results showed that nucleotide sequences of genetic fusion of enterotoxin genes wereconsistent with nucleotide sequences of STa, STb and LT on GeneBank. The desired recombinantprotein was expressed in LB broth with 1mM IPTG substance as inducer.

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